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Home > Past Conferences > 2nd DNA Replication as a Source of DNA Damage Conference

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Past Conference

2nd DNA Replication as a Source of DNA Damage Conference

From Molecules to Human Health

03 Jul 2017 - 06 Jul 2017

Rome, Italy

Synopsis

This conference has now passed however, the following meetings may be of interest;

3rd DNA Replication/Repair Structures and Cancer | 11-15 February 2018 | Cancun, Mexico
DNA & Interacting Proteins as Single Molecules - In Vitro and In Vivo | 21-24 February 2018 | Cancun, Mexico
Nucleic Acids | 13 -16 Feb 2019 | Nassau, Bahamas

The maintenance of genome integrity is critical for the suppression of cancer and premature ageing. Only recently has it become appreciated that DNA replication stress is a crucial driver of genomic instability. The timely progression of replisomes can be disrupted by lesions and secondary structures in the template, by bound proteins and by conflicts with the transcription machinery. A prolonged pause of the replisome then exposes single stranded DNA, which, due to its recombinogenic nature, can lead to genome rearrangements, fragile site expression and cell death. Importantly, some cancers present excessive endogenous levels of replication stress, which can be exploited for their clearance. In this conference, we aim to bring together scientists studying DNA replication and repair, with those interested in how DNA damage can influence cancer and ageing.

Join the conference LinkedIn group to keep up to date with annoucements and latest news concerning the conference.

Key Sessions

  • Pathways for repair of damaged replication forks
  • Systems for site-specific perturbation of replication
  • Chromosome fragility caused by difficult-to-replicate loci – sources and roles of DNA repair proteins
  • Replication perturbation as a driver of tumorigenesis and ageing
  • Exploitation of replication defects in cancer treatment

Confirmed Speakers

Andre Nussenzweig (NIH/NCI)
'GENOME ORGANIZATION DRIVES CHROMOSOME FRAGILITY'

Johannes Walter (Harvard Medical School)
'A ROLE FOR REPLICATION FORK REVERSAL DURING DNA INTERSTRAND CROSSLINK REPAIR'

Jiri Lukas (University of Copenhagen)
'CONFINEMENT AND HERITABILITY OF CELLULAR RESPONSES TO DNA REPLICATION STRESS'

Stephen West  (The Francis Crick Institute)
'UNRESOLVED RECOMBINATION INTERMEDIATES AS A SOURCE OF DNA BREAKS AND CHROMOSOME ABERRATIONS'

Philippe Pasero (CNRS)
'SAMHD1 ACTS AT STALLED FORKS TO PREVENT INFLAMMATION'

John Diffley (The Francis Crick Institute)
'HOW THE REPLICATIVE HELICASE IS LOADED AND ACTIVATED'

Thanos Halazonetis (University of Geneva)
'MECHANISMS OF ONCOGENE-INDUCED DNA REPLICATION STRESS'

Andrew Jackson (University of Edinburgh)
'DONSON ENCODES A NOVEL  REPLICATION FORK PROTECTION FACTOR MUTATED IN MICROCEPHALIC DWARFISM'

Thomas Helleday (Karolinska Institutet)
'IMPORTANCE OF ACCURATE AND TIMELY SUPPLY OF dNTPs FOR REPLICATION AND REPAIR'

Juan Mendez (CNIO)
'NEW MECHANISMS THAT PREVENT DNA OVER-REPLICATION'

Jos Jonkers (Netherlands Cancer Institute)
'GENETIC DETERMINANTS OF TUMOR DEVELOPMENT, THERAPY RESPONSE AND RESISTANCE IN MOUSE MODELS OF BRCA-DEFICIENT BREAST CANCER'

Evi Soutoglou (IGBMC)
'DNA REPAIR PATHWAYS IN DIFFERENT HETEROCHROMATIN STRUCTURES'

Madalena Tarsounas (University of Oxford)
'REPLICATION STRESS TOLERANCE AS A TARGET FOR SELECTIVE ELIMINATION OF BRCA1/2-DEFICIENT CELLS AND TUMOURS'

KJ Patel (University of Cambridge)
'ALDEHYDES AND ENDOGENOUS DNA DAMAGE'

Andres Lopez-Contreras (University of Copenhagen)
'PICH IS ESSENTIAL FOR EARLY EMBRYONIC DEVELOPMENT'

Luis Ignacio Toledo Lazaro (University of Copenhagen)
'EXPLORING THE UNCOUPLING OF ENZYMATIC ACTIVITIES AT REPLICATION FORKS'

Arne Lindqvist (Karolinska Institutet)
'DNA REPLICATION IS AN INTEGRAL COMPONENT OF THE CELL-CYCLE ENGINE'

Wojciech Niedzwiedz (University of Oxford)
'EXD2 NUCLEASE SAFEGUARDS THE GENOME AGAINST REPLICATIVE STRESS'

Philip Zegerman (The Gurdon Institute)
'CHECKPOINT KINASE TARGETTING OF DNA REPLICATION COMPLEXES'

Angelos Constantinou (CNRS)
'DIHYDROPYRIMIDINASE PROTECTS AGAINST DNA REPLICATION STRESS INDUCED BY PYRIMIDINE METABOLITES'

Michael Lisby (University of Copenhagen)
'Mte1/ZGRF1 MAINTAINS GENOME STABILITY DURING REPLICATION STRESS'

Manuel Mendoza (Centre de Regulació Genòmica)
'CELLULAR DETECTION OF CHROMATIN BRIDGES BY THE NoCut ABSCISSION CHECKPOINT'

Target Audience 

The conference aims to target a wide spectrum of scientists interested on how chromosomal rearrangements are generated, and their impact on cancer and ageing: The talks will include studies at various levels ranging from basic molecular biology to clinical studies on human patients. The conference will also appeal to oncologists and pharmaceutical companies with an interest in targeting replication stress as an Achilles’ heel in cancer.

Sponsors

Sponsors

Report

The meeting was chaired again by Profs. Oscar Fernandez-Capetillo (CNIO, Spain and Karolinska Institute, Sweden) and Ian D. Hickson (University of Copenhagen, Denmark).

The invited speakers included several of the worlds leading scientists studying DNA replication stress, DNA repair pathways and the relationship between genomic instability and human disease. The faculty members who presented their latest research findings were:

Andre Nussenzweig (NIH/NCI, Bethesda, USA)
Johannes Walter (Harvard Medical School, USA)
Jiri Lukas (University of Copenhagen, Denmark)
Stephen West (The Francis Crick Institute, London, UK)
Philippe Pasero (CNRS, France)
John Diffley (The Francis Crick Institute, London, UK)
Thanos Halazonetis (University of Geneva, Switzerland)
Andrew Jackson (University of Edinburgh, UK)
Thomas Helleday (Karolinska Institute, Stockholm, Sweden)
Juan Mendez (CNIO, Madrid, Spain)
Jos Jonkers (Netherlands Cancer Institute, Amsterdam)
Evi Soutoglou (IGBMC, France)
Madalena Tarsounas (University of Oxford, UK)
K.J. Patel (University of Cambridge, UK)
Andres Lopez-Contreras (University of Copenhagen, Denmark)
Luis Toledo (University of Copenhagen, Denmark)
Arne Lindqvist (Karolinska Institute, Stockholm, Sweden)
Wojciech Niedzwiedz (University of Oxford, UK)
Philip Zegerman (The Gurdon Institute, Cambridge, UK)
Angelos Constantinou (CNRS, France)
Michael Lisby (University of Copenhagen, Denmark)
Manuel Mendoza (Centre de Regulacio Genomica, Spain)

As with the first meeting in this series, a key feature of the conference was the number of speakers who were selected from the submitted abstracts. These included PhD students/postdocs, as well as several group heads who are early in their career. The fundraising efforts of the organizers permitted many of the selected speakers to have their costs covered by travel awards. There were also two very lively and well attended poster sessions. Sufficient time was set aside for networking and meetings between new and established collaborators. This was facilitated by the pleasant hotel environment.

The combination of a high quality conference venue, the attentive Fusion staff and the Italian summer weather, meant that the meeting had a very comfortable informal atmosphere that facilitated informal discussions, as well as interesting and informative discussion periods after virtually all of the talks. This also encouraged a large proportion of the participants to join in with the discussions.

The conference was very well attended (134 participants), which attests to the growing interest in this field of research – even though several other related conferences are scheduled during 2017. The organizers were satisfied that the conference was a major success.

Programme

MONDAY 03RD JULY 2017

14:00 – 15:00

Registration & Reception

14:00 – 14:45

Group Welcome Lunch

Session #1
Session Chair: Ian Hickson

14:45 – 15:00

Opening Comments by Ian Hickson & Oscar Fernandez-Capetillo

15:00 – 15:25

KJ Patel
University of Cambridge

ALDEHYDES AND ENDOGENOUS DNA DAMAGE

15:25 – 15:40

Rodrigo Bermejo
Center for Biological Research (CIB-CSIC)

COHESIN UBIQUITYLATION AND MOBILIZATION PROMOTES STALLED REPLICATION FORK DYNAMICS AND INTEGRITY

15:40 – 16:05

Manuel Mendoza
Centre for Genomic Regulation

CELLULAR DETECTION OF CHROMATIN BRIDGES BY THE NoCut ABSCISSION CHECKPOINT

16:05 – 16:20

Kara Bernstein
University of Pittsburgh School of Medicine

THE FUNCTION OF THE SHU COMPLEX AND THE RAD51 PARALOGS IN REPAIR OF REPLICATION INTERMEDIATES BY PROMOTION OF RAD51 PRESYNAPTIC FILAMENT ASSEMBLY

16:20 – 16:45

Vincenzo Costanzo
IFOM

MECHANISMS OF REPLICATION FORK PROTECTION

16:45 – 17:00

Jon Houseley
Babraham Institute

ENVIRONMENTAL CHANGE DRIVES ACCELERATED ADAPTATION THROUGH STIMULATED COPY NUMBER VARIATION

17:00 – 17:30

Refreshments

Session #2
Session Chair: KJ Patel

17:30 – 17:55

Ian Hickson
University of Copenhagen

ANALYSIS OF MUTAGENIC EVENTS OCCURRING AT A STALLED REPLICATION FORK

17:55 – 18:10

Pavel Janscak
University of Zurich

RECQ5 HELICASE COOPERATES WITH MUS81 ENDONUCLEASE IN PROCESSING OF STALLED REPLICATION FORKS AT COMMON FRAGILE SITES DURING MITOSIS

18:10 – 18:25

Jennifer Cobb
University of Calgary

NEJ1/XLF REGULATES REPAIR PATHWAY CHOICE BY INHIBITING SGS1/BLM –MEDIATED RESECTION

18:25 – 18:50

Stephen West
Francis Crick Institute

UNRESOLVED RECOMBINATION INTERMEDIATES AS A SOURCE OF DNA BREAKS AND CHROMOSOME ABERRATIONS

18:50 – 19:05

Matthias Dobbelstein
Universitätsmedizin Goettingen

P53 AND Mdm2 AS DETERMINANTS OF CHROMATIN STRUCTURE AND DNA REPLICATION STRESS

19:05 – 19:20

Ana Rojas
Institute of Biomedicine of Seville (IBIS)

COMPUTATIONAL AND EVOLUTIONARY ANALYSES OF NUCS: A NOVEL PROTEIN INVOLVED IN PROKARYOTIC NON-CANONICAL MMR

19:30

Group Dinner

TUESDAY 04TH JULY 2017

07:00 – 09:00

Breakfast

Session #3
Session Chair: Johannes Walter

09:00 – 09:25

Jiri Lukas
Novo Nordisk Foundation Center for Protein Research

CONFINEMENT AND HERITABILITY OF CELLULAR RESPONSES TO DNA REPLICATION STRESS

09:25 – 09:40

Jean-Sébastien Hoffmann
INSERM

REPLICATION STRESS INFLUENCES THE TIMING OF DNA REPLICATION INITIATION OF THE NEXT CELL GENERATION

09:40 – 10:05

Juan Méndez
CNIO

NEW MECHANISMS THAT PREVENT DNA OVER-REPLICATION

10:05 – 10:20

Kevin Hiom
University of Dundee

DHX9 LINKS RNA SPLICING TO THE GENERATION OF REPLICATION BLOCKING R-LOOPS

10:20 – 10:35

Matthias Samwer
Institute for Molecular Biotechnology

A MECHANISM COUNTERACTING MICRONUCLEATION FOR MAINTENANCE OF GENOMIC INTEGRITY

10:35 – 11:05

Refreshments & Group Photo

Session #4
Session Chair: Steve West

11:05 – 11:30

Johannes Walter
Harvard Medical School

A ROLE FOR REPLICATION FORK REVERSAL DURING DNA INTERSTRAND CROSSLINK REPAIR

11:30 – 11:45

Aura Carreira
Institut Curie

A NEW DNA BINDING SITE IN BRCA2 THAT MEDIATES HOMOLOGOUS RECOMBINATION

11:45 – 12:10

Andres Lopez-Contreras
University of Copenhagen

PICH IS ESSENTIAL FOR EARLY EMBRYONIC DEVELOPMENT

12:10 – 12:25

Alberto Ciccia
Columbia University

RESTORATION OF FORK STABILITY IN BRCA1- AND BRCA2-DEFICIENT CELLS

12:25 – 12:50

Angelos Constantinou
IGH-Institute of Human Genetics, CNRS UPR 1142

DIHYDROPYRIMIDINASE PROTECTS AGAINST DNA REPLICATION STRESS INDUCED BY PYRIMIDINE METABOLITES

12:50 – 17:00

Group Lunch & Free Time

Session #5
Session Chair: John Diffley

17:00 – 17:25

Philippe Pasero
CNRS

SAMHD1 ACTS AT STALLED FORKS TO PREVENT INFLAMMATION

17:25 – 17:40

Åsa Ehlén
Institut Curie Research Center

CHARACTERIZATION OF A CLUSTER OF PLK1 PHOSPHORYLATION SITES IN BRCA2 THAT CONTROL MITOTIC ONSET

17:40 – 17:55

Job de Lange
Cancer Center Amsterdam

COHESION WEAKNESS: A UNIQUE VULNERABILITY OF CANCER CELLS

17:55 – 18:10

Lorenza Garribba
University of Copenhagen

INSTABILITY OF A LONG CGG TRIPLET REPEAT IN RESPONSE TO FOLATE DEFICIENCY

18:10 – 18:35

Thanos Halazonetis
University of Geneva

MECHANISMS OF ONCOGENE-INDUCED DNA REPLICATION STRESS

18:35 – 19:05

Refreshments

Session #6
Session Chair: Thanos Halazonetis

19:05 – 19:30

John Diffley
The Francis Crick Institute

HOW THE REPLICATIVE HELICASE IS LOADED AND ACTIVATED

19:30 – 19:45

Stephen Gregory
University of Adelaide

CHROMOSOMAL INSTABILITY CAUSES SUSCEPTIBILITY TO METABOLIC INTERVENTION

19:45 – 20:45

Poster Session & Refreshments

20:45

Group Dinner

WEDNESDAY 05TH JULY 2017

07:00 – 09:00

Breakfast

Session #7
Session Chair: Oscar Fernandez-Capetillo

09:00 – 09:15

Julien Duxin
University of Copenhagen

MECHANISM OF DNA-PROTEIN CROSSLINK PROTEOLYSIS

09:15 – 09:30

Jessica Williams
National Institute of Environmental Health Sciences

THE ROLE OF RNASE H2 IN PROCESSING RIBONUCLEOTIDES INCORPORATED DURING DNA REPLICATION

09:30 – 09:45

Vincent Geli
CRCM

THE NUCLEAR PORE COMPLEX REGULATES REPLICATION STRESS AND TELOMERE RECOMBINATION

09:45 – 10:00

Zee-Fen Chang
National Taiwan University

BCR-ABL TRANSFORMATION LEADS TO REPLICATION STRESS AND INCREASES CELL VULNERABILITY TO THYMIDYLATE KINASE INHIBITION

10:00 – 10:25

Jos Jonkers
Netherlands Cancer Institute

GENETIC DETERMINANTS OF TUMOR DEVELOPMENT, THERAPY RESPONSE AND RESISTANCE IN MOUSE MODELS OF BRCA-DEFICIENT BREAST CANCER

10:25 – 10:55

Refreshments

Session #8
Session Chair: Jiri Lukas

10:55 – 11:20

Andrew Jackson
MRC Human Genetics Unit

DONSON ENCODES A NOVEL  REPLICATION FORK PROTECTION FACTOR MUTATED IN MICROCEPHALIC DWARFISM

11:20 – 11:45

Wojciech Niedzwiedz
Oxford University

EXD2 NUCLEASE SAFEGUARDS THE GENOME AGAINST REPLICATIVE STRESS

11:45 – 12:00

John Tainer
LBL / MD Anderson Cancer Center

FLAP ENDONUCLEASE 1 EMPLOYS PHOSPHATE STEERING TO INSURE SPECIFIC 5´-FLAP INCISION TO PREVENT GENOME INSTABILITY

12:00 – 12:25

Luis Toledo
University of Copenhagen

EXPLORING THE UNCOUPLING OF ENZYMATIC ACTIVITIES AT REPLICATION FORKS

12:25 – 16:20

Group Lunch & Free Time

Session #9
Session Chair: Philippe Pasero

16:20 – 16:45

André Nussenzweig
NIH

GENOME ORGANIZATION DRIVES CHROMOSOME FRAGILITY

16:45 – 17:00

Alessandro Vindigni
Saint Louis University School of Medicine

MRE11 AND EXO1 NUCLEASES DEGRADE REVERSED FORKS IN BRCA-DEFICIENT CELLS AND LEAD TO MUS81-DEPENDENT FORK RESTART

17:00 – 17:25

Arne Lindqvist
Karolinska Institutet

DNA REPLICATION IS AN INTEGRAL COMPONENT OF THE CELL-CYCLE ENGINE

17:25 – 17:40

Katharina Schlacher
MD Anderson Cancer Center

EPIGENETICS-ENABLED MRE11 REPLICATION RESTART BY p53 PROMOTES REPLICATION PATHWAY HOMEOSTASIS TO SUPPRESS OPPORTUNISTIC TRANSCRIPTION REPROGRAMMING

17:40 – 18:05

Michael Lisby
University of Copenhagen

Mte1/ZGRF1 MAINTAINS GENOME STABILITY DURING REPLICATION STRESS

18:05 – 18:20

Olga Murina
MRC Human Genetics Unit

RIBONUCLEOTIDES INCORPORATED DURING GENOME REPLICATION PROVIDE A NOVEL VULNERABILITY TO PARP INHIBITION

18:20 – 18:50

Refreshments

Session #10
Session Chair: Andre Nussenzweig

18:50 – 19:15

Philip Zegerman
Gurdon Institute

CHECKPOINT KINASE TARGETTING OF DNA REPLICATION COMPLEXES

19:15 – 19:40

Madalena Tarsounas
The CR-UK/MRC Oxford Institute for Radiation Oncology, University of Oxford

REPLICATION STRESS TOLERANCE AS A TARGET FOR SELECTIVE ELIMINATION OF BRCA1/2-DEFICIENT CELLS AND TUMOURS

19:40 – 20:40

Poster Session & Refreshments

20:45

*Gala Dinner & Poster Awards*

THURSDAY 06TH JULY 2017

07:00 – 09:15

Breakfast

Session #11
Session Chair: Madalena Tarsounas

09:15 – 09:30

Néstor García-Rodríguez
Institute of Molecular Biology

CHECKPOINT SIGNALING IN RESPONSE TO REPLICATION-BLOCKING LESIONS INITITATES AT DAUGHTER-STRAND GAPS AND ENABLES POSTREPLICATIVE DNA DAMAGE BYPASS

09:30 – 09:55

Thomas Helleday
Karolinska Institutet

IMPORTANCE OF ACCURATE AND TIMELY SUPPLY OF dNTPs FOR REPLICATION AND REPAIR

09:55 – 10:10

Robertus de Bruin
University College London

INCREASED E2F ACTIVITY DURING SPECIFIC PHASES OF THE CELL CYCLE HAS THE POTENTIAL TO INDUCE REPLICATION STRESS VIA DIFFERENT MECHANISMS

10:10 – 10:35

Evi Soutoglou
IGBMC

DNA REPAIR PATHWAYS IN DIFFERENT HETEROCHROMATIN STRUCTURES

10:35 – 10:50

Sabrina Florencia Mansilla
Fundación Instituto leloir

THE ALTERNATIVE POLYMERASE IOTA PROMOTES CHECKPOINT ACTIVATION

10:50 – 11:05

Guillaume Rieunier
University of Oxford

TARGETING THE INSULIN-LIKE GROWTH FACTOR AXIS INDUCES REPLICATION STRESS

11:05 – 11:30

Oscar Fernandez-Capetillo
CNIO / Karolinska Institute

EXPLORING THE ROLE OF THE SMC5/6 COMPLEX IN CANCER AND AGE-RELATED DISEASES

11:30 – 11:45

Closing Comments by Ian Hickson & Oscar Fernandez-Capetillo

Speakers

Invited Speakers

  • Dr. Stephen West
    Senior Group Leader, Francis Crick Institute
  • Dr. John Diffley
    Associate Research Director, The Francis Crick Institute
  • Prof. Johannes Walter
    Professor, Harvard Medical School
  • Professor Thomas Helleday
    Professor, Karolinska Institutet
  • Professor Jiri Lukas
    Managing Director, Novo Nordisk Foundation Center for Protein Research
  • Branch Chief Dr. Andre Nussenzweig
    Branch Chief, Laboratory of Genome Integrity, National Insitutes of Health
  • PhD Angelos Constantinou
    Principal Investigator, IGH-Institute of Human Genetics, CNRS UPR 1142
  • Dr. Manuel Mendoza
    Main Investigator, Centre for Genomic Regulation
  • Prof. Dr. Andrew Jackson
    Group Leader, MRC Human Genetics Unit
  • PhD Andres J Lopez-Contreras
    Group Leader, University of Copenhagen
  • Prof. Jos Jonkers
    Division Head, Netherlands Cancer Institute
  • Prof. Michael Lisby
    Professor, University of Copenhagen
  • Associate Professor Luis Toledo
    Postdoc, Center for Chromosome Stability - 18.1
  • Dr. Philip Zegerman
    Group leader, Gurdon Institute
  • Dr. Wojciech Niedzwiedz
    Group Head, Oxford University
  • PhD. Evi Soutoglou
    Group leader, IGBMC
  • Assist. Prof. Arne Lindqvist
    Group Leader, Karolinska Institutet
  • Professor Thanos Halazonetis
    Professor, University of Geneva
  • Dr. Juan M√©ndez
    Lab Head, Spanish National Cancer Research Centre (CNIO)
  • Dr. Madalena Tarsounas
    CR-UK Senior Group Leader, The CR-UK/MRC Oxford Institute for Radiation Oncology, University of Oxford
  • Dr. Philippe Pasero
    Dr, IGH, CNRS UPR 1142
  • Dr. Ketan Patel
    PI, MRC LMB
  • Dr. Vincenzo Costanzo
    Senior Group Leader, IFOM

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