The advent of single-molecule approaches has revolutionized our understanding of Molecular Biology. Combined advances in light microscopy, microfluidics, atomic force microscopy, and DNA sequencing have opened ways to bypass population averages and study individual molecular events for a variety of cellular processes.
This meeting aims to present the most recent advances in the mechanistic understanding of DNA metabolic processes at the single-molecule level, using both in vivo and in vitro approaches.
Sessions will cover various aspects of:
- DNA replication
- DNA recombination
- DNA repair
- Chromosome dynamics
Each session will be divided into in vitro and in vivo sections to highlight differences and complementary information obtained.
Take advantage of this fantastic opportunity for students! Register an academic at the earlybird rate and bring a student for only $850. Unfortunately, Postdocs are not eligible for this offer. Both registration packages include; accommodation for the 20, 21, 22 February 2020 (on a shared basis for students) and a 24hour all-inclusive food and beverage package for the conference period. Academic registrations must be completed by 8th November 2019. Once registered, please contact Emily Bicknell to obtain a special registration link for your student.
Confirmed Keynote Speaker
Richard Ebright (Rutgers)
RNA POLYMERASE: THE MOLECULAR MACHINE OF TRANSCRIPTION
Confirmed Plenary Speakers
Vincent Croquette (l’ESPCI Paris)
Dorothy Erie (University of North Carolina at Chapel Hill)
INTEGRATIVE SINGLE-MOLECULE STUDIES OF DNA MISMATCH REPAIR INIATION
Antoine van Oijen (University of Wollongong)
SINGLE-MOLECULE STUDIES OF EUKARYOTIC DNA REPLICATION
Maria Spies (The University of Iowa)
MOLECULAR CHOREOGRAPHY OF THE RPA-DNA-RAD52 COMPLEX DURING DNA REPLICATION, RECOMBINATION AND REPAIR
Confirmed Invited Speakers
Ibrahim Cisse (Massachusetts Institute of Technology)
Johan Elf (Uppsala University)
GENOME WIDE BIOPHYSICS OF REPLICATION INITIATION IN E. COLI
Ilya Finkelstein (University of Texas at Austin)
REGULATION OF HUMAN DNA RESECTION
Samir Hamdan (King Abdullah University of Science and Technology)
BUILDING THE REACTION TIMELINE OF FEN1 DURING LAGGING STRAND DNA SYNTHESIS
Rogelio Hernández (synmikro)
UNDERSTANDING PROTEIN MOBILITY IN BACILLUS SUBTILIS BY TRACKING SINGLE MOLECULES
Achillefs Kapanidis (University of Oxford)
DISSECTING TRANSCRIPTION MECHANISMS VIA REAL-TIME IMAGING OF RNA POLYMERASE CONFORMATIONAL CHANGES
Tatiana Karpova (NIH)
Linda Kenney (University of Illinois, Chicago)
Steve Kowalczykowski (University of California, Davis)
WIN, LOOSE, OR DRAW: IMAGING ENCOUNTERS OF INDIVIDUAL MOTOR PROTEINS WITH NUCLEOSOME ARRAYS
Ming Li (Chinese Academy of Sciences)
Tim Lohman (Washington University in St. Louis)
ACTIVATION OF DNA HELICASES BY ACCESSORY FACTORS
Kiyoshi Mizuuchi (NIH)
TWO TYPES OF ParA/B/S PLASMID/CHROMOSOME PARTITION SYSTEMS IN BACTERIA: TWO DIFFERENT PARTITION COMPLEX ARCHITECTURES, A COMMON MECHANISM?
Keir Neuman (NIH)
HOMOLOGY SENSING VIA NON-LINEAR AMPLIFICATION OF SEQUENCE DEPENDENT PAUSING BY RECQ HELICASE
Alexandros Pertsinidis (Memorial Sloan Kettering Cancer Center)
Xiaojun Ren (University of Colorado Denver)
VISUALIZATION OF EPIGENETIC PcG COMPLEXES BY USING LIVE-CELL SINGLE-MOLECULE IMAGING
Ralph Seidel (Universität Leipzig)
DNA TARGET RECOGNITION BY CRISPR-CAS EFFECTOR COMPLEXES
Dave Sherratt (Oxford University)
MukBEF ORGANISES THE E. COLI CHROMOSOME BY FORMING AN AXIAL CORE
Terence Strick (Institut de Biologie de l'École Normale Supérieure)
Gijs Wuite (Vrije Universiteit Amsterdam)
SINGLE MOLECULE MANIPULATION AND IMAGING OF COMPLEX DNA-PROTEIN TRANSACTIONS
Jie Xiao (John Hopkins School of Medicine)
Researchers interested in the study of DNA metabolic events at the single molecule level: both in vitro and in vivo. Processes to be focused on – DNA replication, DNA recombination, DNA repair and transcription. In addition, single cell sequencing/genomics groups will also be targeted.
The single molecule field initially focused on experiments done in vitro. As the field has evolved and new technologies were developed, the understanding of single molecule behaviour in live cells has become increasingly important. The two fields have developed largely separately and by combining these into a single meeting will educate scientists on the value of and difficulties associated with both approaches to understanding key elements in genome maintenance, and the passage of genetic information from DNA to protein.
If you're interested in sponsoring this conference please contact us.